A novel chimeric amine dehydrogenase shows altered substrate specificity compared to its parent enzymes
作者： Bettina R. Bommarius , Martin Schürmann , Andreas S. Bommarius
DOI: 10.1039/C4CC06527A
关键词: Substrate specificity 、 Acetophenone 、 Chemistry 、 Phenylalanine 、 Biochemistry 、 Enzyme 、 Amine dehydrogenase 、 Amination 、 Turn (biochemistry) 、 Leucine
摘要: We created a novel chimeric amine dehydrogenase (AmDH) via domain shuffling of two parent AmDHs (‘L- and F-AmDH’), which in turn had been generated from leucine phenylalanine DH, respectively. Unlike the proteins, AmDH (‘cFL-AmDH’) catalyzes amination acetophenone to (R)-methylbenzylamine adamantylmethylketone adamantylethylamine.
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sci-hub.se 参考文章(10)
Kunishige Kataoka, Harumi Takada, Katsuyuki Tanizawa, Tohru Yoshimura, Nobuyoshi Esaki, Toshihisa Ohshima, Kenji Soda, Construction and characterization of chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase. Journal of Biochemistry. ,vol. 116, pp. 931- 936 ,(1994) , 10.1093/OXFORDJOURNALS.JBCHEM.A124618
Patrick J Baker, Andrew P Turnbull, Svetlana E Sedelnikova, Timothy J Stillman, David W Rice, A role for quaternary structure in the substrate specificity of leucine dehydrogenase Structure. ,vol. 3, pp. 693- 705 ,(1995) , 10.1016/S0969-2126(01)00204-0
C. K. Savile, J. M. Janey, E. C. Mundorff, J. C. Moore, S. Tam, W. R. Jarvis, J. C. Colbeck, A. Krebber, F. J. Fleitz, J. Brands, P. N. Devine, G. W. Huisman, G. J. Hughes, Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture Science. ,vol. 329, pp. 305- 309 ,(2010) , 10.1126/SCIENCE.1188934
Ian R. Wheeldon, Elliot Campbell, Scott Banta, A chimeric fusion protein engineered with disparate functionalities-enzymatic activity and self-assembly. Journal of Molecular Biology. ,vol. 392, pp. 129- 142 ,(2009) , 10.1016/J.JMB.2009.06.075
Janeen L. Vanhooke, James B. Thoden, Norbert M. W. Brunhuber, John S. Blanchard, Hazel M. Holden, Phenylalanine Dehydrogenase from Rhodococcus sp. M4: High-Resolution X-ray Analyses of Inhibitory Ternary Complexes Reveal Key Features in the Oxidative Deamination Mechanism †,‡ Biochemistry. ,vol. 38, pp. 2326- 2339 ,(1999) , 10.1021/BI982244Q
Christopher R Otey, Jonathan J Silberg, Christopher A Voigt, Jeffrey B Endelman, Geethani Bandara, Frances H Arnold, Functional Evolution and Structural Conservation in Chimeric Cytochromes P450: Calibrating a Structure-Guided Approach Chemistry & Biology. ,vol. 11, pp. 309- 318 ,(2004) , 10.1016/J.CHEMBIOL.2004.02.018
Michael J. Abrahamson, John W. Wong, Andreas S. Bommarius, The Evolution of an Amine Dehydrogenase Biocatalyst for the Asymmetric Production of Chiral Amines Advanced Synthesis & Catalysis. ,vol. 355, pp. 1780- 1786 ,(2013) , 10.1002/ADSC.201201030
Miyuki Shimoji, Hequn Yin, LeeAnn Higgins, Jeffrey P. Jones, Design of a Novel P450: A Functional Bacterial−Human Cytochrome P450 Chimera† Biochemistry. ,vol. 37, pp. 8848- 8852 ,(1998) , 10.1021/BI972775Z
Michael J. Abrahamson, Eduardo Vázquez-Figueroa, Nicholas B. Woodall, Jeffrey C. Moore, Andreas S. Bommarius, Development of an amine dehydrogenase for synthesis of chiral amines Angewandte Chemie. ,vol. 51, pp. 3969- 3972 ,(2012) , 10.1002/ANIE.201107813
Hongfang Zhang, George T. Lountos, Chi Bun Ching, Rongrong Jiang, Engineering of glycerol dehydrogenase for improved activity towards 1, 3-butanediol Applied Microbiology and Biotechnology. ,vol. 88, pp. 117- 124 ,(2010) , 10.1007/S00253-010-2735-8