Engineering of glycerol dehydrogenase for improved activity towards 1, 3-butanediol
作者： Hongfang Zhang , George T. Lountos , Chi Bun Ching , Rongrong Jiang
DOI: 10.1007/S00253-010-2735-8
关键词:
摘要: The objective of this study was to use protein engineering techniques to enhance the catalytic activity of glycerol dehydrogenase (GlyDH) on racemic 1, 3-butanediol (1, 3-BDO) for the bioproduction of the important pharmaceutical intermediate 4-hydroxy-2-butanone. Three GlyDH genes (gldA) from Escherichia coli K-12, Salmonella enterica, and Klebsiella pneumoniae MGH78578 were shuffled to generate a random mutagenesis library. The nitroblue tetrazolium/phenazine methosulfate high throughput screening protocol was used …
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