EROD activity and cytochrome P4501A induction in liver and gills of Senegal sole Solea senegalensis from a polluted Huelva Estuary (SW Spain)
作者： Milagrosa Oliva , Carlos Gravato , Lucia Guilhermino , María Dolores Galindo-Riaño , José Antonio Perales
DOI: 10.1016/J.CBPC.2014.07.010
关键词: Estuary 、 Cytochrome 、 Gill 、 Enzyme assay 、 Cellular localization 、 Staining 、 Molecular biology 、 Immunohistochemistry 、 Endothelium 、 Anatomy 、 Biology
摘要: EROD activity and induction cytochrome P4501A in liver gills of Senegal sole, Solea senegalensis, from a heavy metal PAH polluted estuary, was studied. Liver gill CYP1A catalytic assessed at the enzyme level-measured as 7-ethoxyresorufin-O-deethylase cellular localization studied using immunohistochemistry. correlated with phenanthrene-type metabolites copper concentrations water. Strong occurrence observed acinar pancreatic cells, duct epithelium vascular system endothelium negative/rare were hepatocytes sinusoidal endothelium. In gills, showed significant correlation different fractions metals sediment but no between PAHs. Strongly positive associated staining endothelia primary filament cells moderate pillar observed. The results substantiated utility measurement biomarkers for use by aquatic toxicologists indicate that assays immunohistochemical appear to be sensitive kinds pollutants being both methods recommended monitoring programs.
uca.es
sci-hub.se 参考文章(73)
Carmen Sarasquete, Helmut Segner, Juana M. Arellano, Juan B. Ortiz-Delgado, Histopathological alterations, EROD activity, CYP1A protein and biliary metabolites in gilthead seabream Sparus aurata exposed to benzo(a)pyrene. Histology and Histopathology. ,vol. 22, pp. 417- 432 ,(2007) , 10.14670/HH-22.417
P L Goering, Lead-protein interactions as a basis for lead toxicity. Neurotoxicology. ,vol. 14, pp. 45- 60 ,(1993)
Quig D, Cysteine metabolism and metal toxicity. Alternative medicine review : a journal of clinical therapeutic. ,vol. 3, pp. 262- 270 ,(1998)
David W. Martin, Harold Anthony Harper, Harper's review of biochemistry Lange Medical Publications , Maruzen [distributor]. ,(1983)
Richard T. Mayer, M. Danny Burke, ETHOXYRESORUFIN: DIRECT FLUORIMETRIC ASSAY OF A MICROSOMAL O-DEALKYLATION WHICH IS PREFERENTIALLY INDUCIBLE BY 3-METHYLCHOLANTHRENE Drug Metabolism and Disposition. ,vol. 2, pp. 583- 588 ,(1974)
Jasmine Nahrgang, Martina Jönsson, Lionel Camus, EROD activity in liver and gills of polar cod (Boreogadus saida) exposed to waterborne and dietary crude oil Marine Environmental Research. ,vol. 70, pp. 120- 123 ,(2010) , 10.1016/J.MARENVRES.2010.02.003
G.H. Lu, Y. Ji, H.Z. Zhang, H. Wu, J. Qin, C. Wang, Active biomonitoring of complex pollution in Taihu Lake with Carassius auratus. Chemosphere. ,vol. 79, pp. 588- 594 ,(2010) , 10.1016/J.CHEMOSPHERE.2010.01.053
L.R. Vieira, C. Gravato, A.M.V.M. Soares, F. Morgado, L. Guilhermino, Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: Linking biomarkers to behaviour Chemosphere. ,vol. 76, pp. 1416- 1427 ,(2009) , 10.1016/J.CHEMOSPHERE.2009.06.005
M. Oliveira, M. Pacheco, M.A. Santos, Cytochrome P4501A, genotoxic and stress responses in golden grey mullet (Liza aurata) following short-term exposure to phenanthrene Chemosphere. ,vol. 66, pp. 1284- 1291 ,(2007) , 10.1016/J.CHEMOSPHERE.2006.07.024
Aldo Viarengo, Biochemical effects of trace metals Marine Pollution Bulletin. ,vol. 16, pp. 153- 158 ,(1985) , 10.1016/0025-326X(85)90006-2