Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes.

摘要: Abstract Although the molecular basis of allergenicity remains to be fully elucidated, ability allergens elicit allergic responses is at least partly attributed their proteolytic activity. Protease-activated receptor-2 (PAR-2) a G protein-coupled receptor that activated by site-specific proteolysis serine proteases and known mediate inflammatory processes in various tissues. In this study, we investigated effects trypsin, major protease, human PAR-2 agonist peptide (SLIGKV-NH 2 ) on proinflammatory cytokine chemokine gene expression canine keratinocyte cell line CPEK. The mRNA protein CPEK cells was detected RT-PCR Western blotting, respectively. localization examined immunofluorescence. levels cytokines chemokines were quantified real-time RT-PCR. free intracellular Ca 2+ concentration measured using -sensitive fluorescent dye. constitutively expressed protein. Stimulation with trypsin induced significant upregulation tumor necrosis factor alpha (TNF-α, P internalization. These results suggest activation can augment keratinocytes, it may initiate inflammation through activity atopic dermatitis.