Morphologic and quantitative assessment of CD20+ B cell infiltrates in rheumatoid arthritis-associated nonspecific interstitial pneumonia and usual interstitial pneumonia.

摘要: Objective B lymphocytes are emerging as important elements in the events leading to joint destruction rheumatoid arthritis (RA). However, B have not been studied (RA)–associated lung disease. We performed a morphologic and quantitative analysis of plasma cells RA-associated interstitial pneumonia (IP) comparison with idiopathic IP normal lungs. Methods Open-lung biopsy specimens from patients (n = 18), 21), control subjects 11) were stained antibodies CD20 CD138. Morphologic patterns characterized staining was quantified using computer-assisted image analysis. Results In IP, marked follicular cell hyperplasia detected, which limited almost entirely peribronchiolar lymphoid aggregates. Plasma also present large numbers, but showed more diffuse tissue infiltration. Quantification demonstrated higher cellularity (median 2.0%, interquartile range [IQR] 1.0–5.7) compared (0.9%, IQR 0.5–2.1). Control significantly smaller number both diseases (0.4%, 0.1–1.3). In RA who smokers those male, proportion CD20+ areas further increased 4.3% (IQR 1.0–5.8) 3.9% 0.7–6.9), respectively. Conclusion We significant IP. The differences between imply differential emphasis cell–mediated mechanisms 2 despite radiologic histologic similarities provide rationale for studying functional aspects involvement pathogenesis