Impact of repeated intravenous bone marrow mesenchymal stem cells infusion on myocardial collagen network remodeling in a rat model of doxorubicin-induced dilated cardiomyopathy

摘要: Bone marrow mesenchymal stem cells (MSCs) transplantation improved cardiac function and reduced myocardial fibrosis in both ischemic non-ischemic cardiomyopathies. We evaluated the effects of repeated peripheral vein injection MSCs on collagen network remodeling TGF-β1, AT1, CYP11B2 (aldosterone synthase) gene expressions a rat model doxorubicin (DOX)-induced dilated cardiomyopathy (DCM). Thirty-eight out 53 SD rats survived at 10 weeks post-DOX (2.5 mg/kg/week for 6 weeks, i.p.) were divided into DCM blank (without treatment, n = 12), placebo (intravenous tail 0.5 mL serum-free culture medium every other day ten times, 13), plus group 5 × 10(6) dissolved 13). Ten untreated served as normal controls. At 20 after DOX injection, echocardiography, content, types I III collagen, compared among groups. 8 (67%) group, 9 (69%) while 13 (100 %) group. Left ventricular end-diastolic diameter was significantly higher ejection fraction lower groups to control rats, which (all p < 0.05 vs. groups). Moreover, volume fraction, mRNA CYP11B2, I/III ratio all 0.05). Repeated intravenous could improve by attenuating possibly through downregulating renin-angiotensin-aldosterone system DOX-induced rats.