Overexpression of an ectopic H19 gene enhances the tumorigenic properties of breast cancer cells

摘要: The maternally expressed H19 gene is transcribed as an untranslated RNA that serves a riboregulator. We have previously reported this transcript accumulates in epithelial cells approximately 10% of breast cancers. To gain further insight on how the overexpression affects phenotype human cells, we investigated oncogenic potential was abundantly from MDA-MB-231 cancer stably transfected with genomic sequence gene. amount did not affect cell proliferation capacity, timing cycle phases or anchorage-dependent ability H19-transfected clones vitro. But anchorage-independent growth assays H19-recombined formed more and larger colonies soft-agar versus control cells. explore phenotypic change, analysed tumour development after subcutaneous injection into scid mice. Results showed promotes progression. These data support hypothesis overload associated exhibiting higher tumorigenic phenotypes therefore conclude has properties