Characterization of the complete mitochondrial genome of Metastrongylus salmi (M. salmi) derived from Tibetan pigs in Tibet, China.

作者: Kun Li , Muhammad Shahzad , Hui Zhang , Khalid Mehmood , Xiong Jiang

DOI: 10.1515/AP-2018-0032

关键词: Parasite hostingTransfer RNAMetastrongylus salmiBiologyGeneMitochondrial DNAGenomePhylogenetic treeGeneticsPopulation

摘要: The present study was designed to determine and analyze the mt genomes of Metastrongylus salmi (M. salmi), reveal phylogenetic relationships this parasite using DNA sequences. Results showed that complete genome M. 13722 bp containing 12 protein-coding genes (cox1-3, nad1-6, nad4L, atp6 cytb), 22 transfer RNA genes, 2 ribosomal (rrnL rrnS). overall A+T content 73.54% nucleotide composition A (23.52%), C (6.14%), G (19.60%), T (50.02%), N (UCAG) (0.73%). total 4237 amino acids are encoded from Tibetan isolates genomes. ATA predicted as most common starting codon with 41.7% (5/12 protein genes); 11 were found have a TAG or TAA translation termination codon. By clustering together trees Austrian salmi, isolated pigs be highly homological stemmed one. This information provides meaningful insights into position China isolate represents useful resource for selecting molecular markers diagnosis population studies.

参考文章(38)
Young Bong Choi, John Nicholas, Bim Nuclear Translocation and Inactivation by Viral Interferon Regulatory Factor PLOS Pathogens. ,vol. 6, pp. 1- 1 ,(2010) , 10.1371/JOURNAL.PPAT.1001031
Diana Gassó, Luca Rossi, Gregorio Mentaberre, Encarna Casas, Roser Velarde, Pawel Nosal, Emmanuel Serrano, Joaquim Segales, Pedro Fernandez-Llario, Carles Feliu, None, An identification key for the five most common species of Metastrongylus. Parasitology Research. ,vol. 113, pp. 3495- 3500 ,(2014) , 10.1007/S00436-014-4001-Y
Eugene Koonin, Roman Tatusov, Michael Galperin, Mikhail Rozanov, David Lipman, A Genomic Perspective on Protein Families Science. ,vol. 278, pp. 631- 637 ,(1997) , 10.1126/SCIENCE.278.5338.631
Nian-Zhang Zhang, Dong-Hui Zhou, Si-Yang Huang, Meng Wang, Xin-Chun Shi, Danba Ciren, Xing-Quan Zhu, Seroprevalence and risk factors associated with Haemophilus parasuis infection in Tibetan pigs in Tibet Acta Tropica. ,vol. 132, pp. 94- 97 ,(2014) , 10.1016/J.ACTATROPICA.2013.12.021
Kevin G. Helfenbein, Wesley M. Brown, Jeffrey L. Boore, The Complete Mitochondrial Genome of the Articulate Brachiopod Terebratalia transversa Molecular Biology and Evolution. ,vol. 18, pp. 1734- 1744 ,(2001) , 10.1093/OXFORDJOURNALS.MOLBEV.A003961
Min Hu, Neil B Chilton, Robin B Gasser, The mitochondrial genome of Strongyloides stercoralis (Nematoda) – idiosyncratic gene order and evolutionary implications International Journal for Parasitology. ,vol. 33, pp. 1393- 1408 ,(2003) , 10.1016/S0020-7519(03)00130-9
Minoru Kanehisa, A database for post-genome analysis Trends in Genetics. ,vol. 13, pp. 375- 376 ,(1997) , 10.1016/S0168-9525(97)01223-7
G. Marruchella, B. Paoletti, R. Speranza, G. Di Guardo, Fatal bronchopneumonia in a Metastrongylus elongatus and Porcine circovirus type 2 co-infected pig. Research in Veterinary Science. ,vol. 93, pp. 310- 312 ,(2012) , 10.1016/J.RVSC.2011.05.016
Guang-Hui Zhao, Bing Hu, Wen-Yu Cheng, Yan-Qing Jia, Hong-Mei Li, San-Ke Yu, Guo-Hua Liu, The complete mitochondrial genomes of Oesophagostomum asperum and Oesophagostomum columbianum in small ruminants Infection, Genetics and Evolution. ,vol. 19, pp. 205- 211 ,(2013) , 10.1016/J.MEEGID.2013.07.018