Possible clearance of transfusion-acquired nef/LTR-deleted attenuated HIV-1 infection by an elite controller with CCR5 Δ32 heterozygous and HLA-B57 genotype.

摘要: Abstract Background Subject C135 is one of the members Sydney Blood Bank Cohort, infected in 1981 through transfusion with attenuated nef/3′ long terminal repeat (LTR)-deleted HIV-1, and has maintained undetectable plasma viral load steady CD4 cell count, absence therapy. Uniquely, combines five factors separately associated control viraemia: nef/LTR-deleted HLA-B57, HLA-DR13, heterozygous CCR5 Δ32 genotype vigorous p24-stimulated peripheral blood mononuclear (PBMC) proliferation. Therefore, we studied detail burden immunological responses this individual. Methods PBMC gut lymph node biopsy samples were analysed for proviral HIV-1 DNA by real-time nested PCRs, nef/LTR alleles PCR. HIV-specific antibodies Western blotting, CD4+ CD8+ T lymphocyte measured proliferation cytokine production vitro. Results from 1996, but not since, showed amplification nef gross deletions. Infectious was never recovered. Proviral detected recent or samples. a consistently weak antibody response substantial proliferative to previously described HLA-DR13-restricted epitope p24 vitro, which augmented an immunodominant HLA-B57-restricted p24, while his cells show reduced levels CCR5. Conclusions C135's early PCR results are consistent limited infection poorly replicating strain HIV-1. With gag-specific CD8 helper responses, appears have cleared 37 years after transfusion.