Biomimetic Macroporous PCL Scaffolds for Ex Vivo Expansion of Cord Blood-Derived CD34+Cells with Feeder Cells Support
作者： Xiuwei Pan , Qiong Sun , Yuanhao Zhang , Haibo Cai , Yun Gao
关键词: CD34 、 Mesenchyme 、 Chemistry 、 Extracellular matrix 、 Cord blood 、 Anatomy 、 Haematopoiesis 、 Stromal cell 、 Cell biology 、 Stem cell 、 In vivo 、 Biotechnology 、 Materials Chemistry 、 Bioengineering 、 Polymers and Plastics 、 Biomaterials
摘要: Ex vivo expansion of hematopoietic stem cells (HSCs) with most current methods can hardly satisfy clinical application requirement. While in vivo, HSCs efficiently self-renew niche where they interact 3D extracellular matrix and stromal cells. Therefore, co-cultures CD34+ mesenchyme derived from human amniotic membrane (hAMSCs) on the basis biomimetic macroporous three-dimensional (3D) poly(e-caprolactone) (PCL) scaffolds are developed, hAMSCs applied to mimic structural cellular microenvironment HSCs. The influence scaffolds, feeder cells, contact manners stemness maintenance is investigated this protocol. Biomimetic scaffolds-dependent effectively promote cells; meanwhile, indirect superior direct contact. combination represents a new strategy for achieving clinical-scale ex
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