A practical approach to diagnostic Ig/TCR clonality evaluation in clinical pathology

作者： Patricia J. T. A. Groenen , Annemiek van Raaij , Margarethe C. van Altena , Paul M. Rombout , J. M. Han van Krieken

关键词: Heteroduplex 、 Gene rearrangement 、 Clinical pathology 、 Immunology 、 T-cell receptor 、 Multiplex 、 Biology 、 Computational biology

摘要: The BIOMED-2 group developed and approved a set of multiplex Ig T cell receptor PCR primers successfully applied these to different well-defined WHO lymphoma entities with unprecedented high frequencies malignant cases showing clonality. This approach has now become worldwide standard clonality testing in lymphoproliferations. While the assessment by GeneScan and/or heteroduplex analysis relatively easy perform, evaluation obtained gene rearrangement patterns can be difficult. In this review, we will address specific aspects testing, concerning both practical phase as patterns, which help overcome problems that encountered routine diagnostic setting.

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A practical approach to diagnostic Ig/TCR clonality evaluation in clinical pathology

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A practical approach to diagnostic Ig/TCR clonality evaluation in clinical pathology

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