Physiological evidence on the nature of the repressor of alkaline phosphatase synthesis in Escherichia coli

摘要: The synthesis of alkaline phosphatase has been studied in a mutant Escherichia coli which repression by inorganic phosphate is temperature-dependent. When added back to derepressed cultures this strain, the restoration proceeds more rapidly lower temperature cultivation. excess shown be biosynthetic process which: (1) dependent on presence required amino acid; (2) completely blocked chloramphenicol; (3) concentrations 5-fiuorouracil substantially inhibit RNA but only slightly protein synthesis; (4) absence thymine for DNA synthesis. These properties indicate that repressor whose rate varies inversely with strain. Control experiments fully constitutive rule out possibility inhibitory conditions employed affect unregulated phosphatase. apparent requirement de novo after small amount growth indicates metabolically unstable.