Quantitation of Candida albicans Ergosterol Content Improves the Correlation between In Vitro Antifungal Susceptibility Test Results and In Vivo Outcome after Fluconazole Treatment in a Murine Model of Invasive Candidiasis

摘要: MIC end point determination for the most commonly prescribed azole antifungal drug, fluconazole, can be complicated by “trailing” growth of organism during susceptibility testing National Committee Clinical Laboratory Standards approved M27-A broth macrodilution method and its modified microdilution format. To address this problem, we previously developed sterol quantitation (SQM) in vitro fluconazole susceptibility, which measures cellular ergosterol content rather than inhibition after exposure to fluconazole. determine if SQM MICs correlated better with vivo outcome MICs, used a murine model invasive candidiasis analyzed capacity treat infections caused C. albicans isolates were trailers (M27-A at 24 48 h, ≤1.0 ≥64 μg/ml, respectively; MIC, μg/ml), as well those sensitive μg/ml) resistant μg/ml; 54 μg/ml). Compared untreated controls, therapy increased survival mice infected isolate both trailing but did not increase isolate. These results indicate that is more predictive give unclear points due