Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism.

摘要: A method for the rapid and quantitative separation of glycerophosphocholine, choline phosphate upon ion-exchange columns is described. The has been utilized to examine stimulation phosphatidylcholine breakdown in quiescent Swiss 3T3 cells response bombesin 12-O-tetradecanoylphorbol 13-acetate (TPA). stimulated generation shown precede that phosphate, with no effect glycerophosphocholine levels; but was attenuated which protein kinase C activity down-regulated. results thus suggest either or TPA activates phospholipase D-catalysed by a common mechanism involving activation C.