Thermal dissociation of ions limits the degree of the gas-phase H/D exchange at the atmospheric pressure.

摘要: We present the application of extended desolvating capillaries for increasing degree gas-phase hydrogen/deuterium exchange reaction at atmospheric pressure. The use results in increase time that ions spend high pressure region, what leads to significant improvement efficiency reaction. For small protein ubiquitin, it was observed same temperature, number exchanges increases with decrease charge state so lowest can twice hydrogen than highest one. With difference decreases, and eventually, equalizes all states. value this temperature corresponding depend on geometric parameters capillary. Further thermal dissociation ion. b/y fragments are identical those produced by collision-induced performed ion trap. Copyright © 2017 John Wiley & Sons, Ltd.