Detection of vancomycin-resistant Enterococcus faecalis ST6-vanB2 and E. faecium ST915-vanA in faecal samples of wild Rattus rattus in Spain.

摘要: Abstract The detection of vancomycin-resistant-enterococci (VRE) among wild animals represents a worrisome public health concern. objectives the study were to determine possible presence VRE in faecal samples small mammals Spain, characterize vancomycin resistance mechanisms and genetic lineages recovered isolates know diversity enterococcal species these animals. A total 155 from inoculated Slanetz–Bartley agar supplemented or not with (Van-SB/SB plates). antimicrobial susceptibility profile 12 antimicrobials 20 genes was analyzed. structure Tn1546 gelE, cylA, asa, esp hyl studied. Multilocus-sequence-typing (MLST) technique also performed. Van-SB plates 11 samples. Two contained vanB2-positive E. faecalis lineage ST6, which showed multiresistance phenotype harboured virulence gelE asa. One sample vancomycin-resistant faecium isolate new ST915, vanA gene included into (truncated IS1542 IS1216 elements). vanB2 obtained Rattus rattus. remaining eight VRE-positive intrinsic vancomycin-resistance mechanisms: casseliflavus (n = 5) gallinarum (n = 3). hundred forty-seven vancomycin-susceptible-enterococcal SB plates, most frequent detected species. This is first report vanB2-containing enterococci