Cytosolic-free calcium elevation in Trypanosoma cruzi is required for cell invasion.

摘要: To replicate, the trypomastigote form of Trypanosoma cruzi must invade host cells. Since a role for Ca2+ in process cell invasion by several intracellular parasites has been postulated, changes concentration T. trypomastigotes and tissue culture L6E9 myoblasts during their interaction were studied at single level using digital imaging fluorescence microscopy or suspensions spectrophotometry. An increase cytosolic was detected after association with myoblasts. mobilization cells also upon contact either grown coverslips exposed to trypomastigotes. Pretreatment chelators quin 2 (50 microM) bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA, 50 decreased trypomastigotes' approximately 40 63%, respectively, thus indicating that an is required addition