Poly(N-isopropylacrylamide)-graft-polypropylene membranes containing adsorbed antibody for cell separation.
作者： Aiko Okamura , Midori Itayagoshi , Taeko Hagiwara , Manae Yamaguchi , Toshiyuki Kanamori
DOI: 10.1016/J.BIOMATERIALS.2004.04.028
关键词: Polymerization 、 Cell 、 Antibody 、 Desorption 、 Monoclonal antibody 、 Transfection 、 Membrane 、 Polymer chemistry 、 Adsorption 、 Materials science
摘要: We developed a novel selective cell-separation method based on using poly(N-isopropylacrylamide)-graft-polypropylene (PNIPAAm-g-PP) membrane containing adsorbed monoclonal antibody specific to the target cell. This was prepared by plasma-induced polymerization and soaking in an solution at 37 degrees C. Poly(N-isopropylacrylamide) has thermoresponsive phase transition: 32 C water-insoluble (hydrophobic) water-soluble (hydrophilic) states interconvert. Adsorption of onto PNIPAAm-g-PP its desorption 4 verified fluorescence-microscopy after it fluorescein-conjugated goat anti-mouse IgG phosphate-buffered saline. membranes CD80 preferentially captured mouse-CD80 transfected cells compared with lacking or CD86 antibody. Detachment from facilitated washing because transition PNIPAAm. With this method, mouse CD80- CD86-transfected were enriched 1:1 cell suspension 72% 66%, simply high yield.
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