Hepatocyte growth factor/scatter factor induces tyrosine phosphorylation of focal adhesion kinase (p125FAK) and promotes migration and invasion by oral squamous cell carcinoma cells.
作者： K Matsumoto , K Matsumoto , T Nakamura , R H Kramer
DOI: 10.1016/S0021-9258(18)31767-8
关键词: Tyrosine phosphorylation 、 Hepatocyte growth factor 、 Vinculin 、 Platelet-derived growth factor receptor 、 PTK2 、 Paxillin 、 Tyrosine kinase 、 Focal adhesion 、 Cell biology 、 Biology
摘要: Fibroblasts or their conditioned medium stimulated invasion by squamous cell carcinoma cells. The fibroblast-derived activity responsible for increased is the hepatocyte growth factor/scatter factor (HGF/SF), a ligand c-Met receptor. HGF/SF migration of cells on various extracellular matrix substrates but did not alter adhesion efficiency nor integrin expression. motility in two step process: initially spread rapidly and formed focal adhesions, then they disassembled these condensations, which was followed locomotion. adhesions contained vinculin, p125FAK, beta 1 integrin, phosphotyrosine. Within minutes after exposure to HGF/SF, proteins 125 145 kDa showed elevated tyrosine phosphorylation were identified as p125FAK c-Met, respectively. Gradual loss coincided with disruption conversion motile phenotype. HGF/SF-mediated inhibited kinase inhibitor, herbimycin A, also blocked spreading migratory response. These results indicate that triggers through initial recruiting integrins, cytoskeletal proteins, into dependent activity.
sci-hub.st 参考文章(46)
R. P. Narsimhan, E. Medico, P. M. Comoglio, M. F. Di Renzo, S. Giordano, M. Olivero, P. Gaglia, P. Zara, S. Bretti, Expression of the Met/HGF receptor in normal and neoplastic human tissues. Oncogene. ,vol. 6, pp. 1997- 2003 ,(1991)
Cheng Yf, Kramer Rh, Ramos Dm, Role of laminin-binding integrin in the invasion of basement membrane matrices by fibrosarcoma cells. Invasion & Metastasis. ,vol. 11, pp. 125- ,(1991)
M.M. Hamawy, S.E. Mergenhagen, R.P. Siraganian, Tyrosine phosphorylation of pp125FAK by the aggregation of high affinity immunoglobulin E receptors requires cell adherence. Journal of Biological Chemistry. ,vol. 268, pp. 6851- 6854 ,(1993) , 10.1016/S0021-9258(18)53114-8
A. Graziani, D. Gramaglia, P. dalla Zonca, P.M. Comoglio, Hepatocyte growth factor/scatter factor stimulates the Ras-guanine nucleotide exchanger Journal of Biological Chemistry. ,vol. 268, pp. 9165- 9168 ,(1993) , 10.1016/S0021-9258(18)98327-4
L Kornberg, H.S. Earp, J.T. Parsons, M Schaller, R.L. Juliano, Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase. Journal of Biological Chemistry. ,vol. 267, pp. 23439- 23442 ,(1992) , 10.1016/S0021-9258(18)35853-8
P. Santala, J. Heino, Regulation of integrin-type cell adhesion receptors by cytokines. Journal of Biological Chemistry. ,vol. 266, pp. 23505- 23509 ,(1991) , 10.1016/S0021-9258(18)54526-9
I Zachary, J Sinnett-Smith, E Rozengurt, Bombesin, vasopressin, and endothelin stimulation of tyrosine phosphorylation in Swiss 3T3 cells. Identification of a novel tyrosine kinase as a major substrate. Journal of Biological Chemistry. ,vol. 267, pp. 19031- 19034 ,(1992) , 10.1016/S0021-9258(18)41733-4
S. Rankin, E. Rozengurt, Platelet-derived growth factor modulation of focal adhesion kinase (p125FAK) and paxillin tyrosine phosphorylation in Swiss 3T3 cells. Bell-shaped dose response and cross-talk with bombesin. Journal of Biological Chemistry. ,vol. 269, pp. 704- 710 ,(1994) , 10.1016/S0021-9258(17)42406-9
M D Schaller, J D Hildebrand, J D Shannon, J W Fox, R R Vines, J T Parsons, Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2-dependent binding of pp60src. Molecular and Cellular Biology. ,vol. 14, pp. 1680- 1688 ,(1994) , 10.1128/MCB.14.3.1680
T. Takehara, T. Nakamura, J.S. Rubin, W.G. Taylor, R.A. Furlong, Comparison of biological and immunochemical properties indicates that scatter factor and hepatocyte growth factor are indistinguishable. Journal of Cell Science. ,vol. 100, pp. 173- 177 ,(1991)