Experimental and clinicopathologic studies on the function of the HGF receptor in human colon cancer metastasis.

摘要: The organ-specific metastasis characterizes several human cancers, including colon carcinoma, a disease that frequently involves metastases in the liver. data on molecular mechanisms of liver would therefore be highly useful for prognostic purposes. Although upregulation/amplification hepatocyte growth factor (HGF) receptor, c-met, has been observed cancer metastasis, actual functional significance feature metastatization is not yet known. We have used three carcinoma cell lines (HT29, HT25 and WiDr), characterized by different metastatic potentials SCID mice, to analyze expression c-met biological effects HGF. found HGF induces scattering vitro liver-metastatic (HT25 WiDr) only at doses which are non-mitogenic (1–20 ng/ml). Analysis revealed express authentic gene protein material, unlike non-metastatic HT29 line, expresses c-terminal cytoplasmic domain β-chain. Interestingly, was localized substrate-attached peripheral membrane partially colocalized with phosphotyrosine-proteins cells when kept fibronectin. On other hand, we analyzed 86 primary cancers Dukes' B (invasive but non-metastatic) C lymph node metastatic) stages. Western blotting proteins isolated from tumor tissues immunohistochemical control study paraffin samples third these cases (25/86) all indicated significant upregulation glands compared stages (P<0.001 P<0.05, respectively). Since two differ involvement regional nodes invasion depth, clinicopathological our experimental findings further support notion can considered as marker potential due its generation motility signal.