Cytotoxic, genotoxic and pro-inflammatory effects of zinc oxide nanoparticles in human nasal mucosa cells in vitro
作者： Stephan Hackenberg , Agmal Scherzed , Antje Technau , Michael Kessler , Katrin Froelich
DOI: 10.1016/J.TIV.2011.01.003
关键词: In vitro 、 Interleukin 8 、 Cytotoxic T cell 、 Molecular biology 、 Intracellular 、 Genotoxicity 、 Zinc 、 Pathology 、 Comet assay 、 Mucous membrane of nose 、 Chemistry
摘要: Abstract Despite increasing application of zinc oxide nanoparticles (ZnO-NPs) for industrial porpuses, data about potential toxic properties is contradictory. The current study focused on the cyto- and genotoxicity ZnO-NPs in comparison to ZnO powder primary human nasal mucosa cells cultured air–liquid interface. Additionally, IL-8 secretion as a marker pro-inflammatory effects was measured. Particle morphology intracellular distribution were evaluated by transmission electron microscopy (TEM). transferred into cytoplasm 10% cells, whereas an intranuclear could only be observed 1.5%. While no or seen dimethylthiazolyl-diphenyl-tetrazolium-bromide (MTT) test, trypan blue exclusion single-cell microgel electrophoresis (comet) assay, cytotoxic shown at ZnO-NP concentration 50 μg/ml (P
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sci-hub.se 参考文章(43)
I. A. Olivotto, P. L. Olive, R. E. Durand, S. M. Jackson, J. Le Riche, Gel Electrophoresis of Individual Cells to Quantify Hypoxic Fraction in Human Breast Cancers Cancer Research. ,vol. 53, pp. 733- 736 ,(1993)
Tobias J. Brunner, Peter Wick, Pius Manser, Philipp Spohn, Robert N. Grass, Ludwig K. Limbach, Arie Bruinink, Wendelin J. Stark, In Vitro Cytotoxicity of Oxide Nanoparticles: Comparison to Asbestos, Silica, and the Effect of Particle Solubility† Environmental Science & Technology. ,vol. 40, pp. 4374- 4381 ,(2006) , 10.1021/ES052069I
Weidong Wu, James M. Samet, David B. Peden, Philip A. Bromberg, Phosphorylation of p65 is required for zinc oxide nanoparticle-induced interleukin 8 expression in human bronchial epithelial cells. Environmental Health Perspectives. ,vol. 118, pp. 982- 987 ,(2010) , 10.1289/EHP.0901635
Vyom Sharma, Ritesh K. Shukla, Neha Saxena, Devendra Parmar, Mukul Das, Alok Dhawan, DNA damaging potential of zinc oxide nanoparticles in human epidermal cells. Toxicology Letters. ,vol. 185, pp. 211- 218 ,(2009) , 10.1016/J.TOXLET.2009.01.008
David M Brown, Claire Dickson, Paul Duncan, Furat Al-Attili, Vicki Stone, Interaction between nanoparticles and cytokine proteins: impact on protein and particle functionality. Nanotechnology. ,vol. 21, pp. 215104- ,(2010) , 10.1088/0957-4484/21/21/215104
Eric K. Dufour, Tirukalikundram Kumaravel, Gerhard J. Nohynek, David Kirkland, Hervé Toutain, Clastogenicity, photo-clastogenicity or pseudo-photo-clastogenicity: Genotoxic effects of zinc oxide in the dark, in pre-irradiated or simultaneously irradiated Chinese hamster ovary cells. Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 607, pp. 215- 224 ,(2006) , 10.1016/J.MRGENTOX.2006.04.015
Qi Qi, Tong Zhang, Qingjiang Yu, Rui Wang, Yi Zeng, Li Liu, Haibin Yang, Properties of humidity sensing ZnO nanorods-base sensor fabricated by screen-printing Sensors and Actuators B-chemical. ,vol. 133, pp. 638- 643 ,(2008) , 10.1016/J.SNB.2008.03.035
Michael Buehrlen, Ulrich A. Harréus, Fernando Gamarra, Rudolf Hagen, Norbert H. Kleinsasser, Cumulative genotoxic and apoptotic effects of xenobiotics in a mini organ culture model of human nasal mucosa as detected by the alkaline single cell microgel electrophoresis assay and the annexin V-affinity assay Toxicology Letters. ,vol. 169, pp. 152- 161 ,(2007) , 10.1016/J.TOXLET.2007.01.005
Fen Xu, Peng Zhang, Alexandra Navrotsky, Zhong-Yong Yuan, Tie-Zhen Ren, Matej Halasa, Bao-Lian Su, HIERARCHICALLY ASSEMBLED POROUS ZNO NANOPARTICLES:? SYNTHESIS, SURFACE ENERGY, AND PHOTOCATALYTIC ACTIVITY Chemistry of Materials. ,vol. 19, pp. 5680- 5686 ,(2007) , 10.1021/CM071190G
Peter Bihari, Minnamari Vippola, Stephan Schultes, Marc Praetner, Alexander G Khandoga, Christoph A Reichel, Conrad Coester, Timo Tuomi, Markus Rehberg, Fritz Krombach, Optimized dispersion of nanoparticles for biological in vitro and in vivo studies Particle and Fibre Toxicology. ,vol. 5, pp. 14- 14 ,(2008) , 10.1186/1743-8977-5-14