Deep metagenomics examines the oral microbiome during dental caries, revealing novel taxa and co-occurrences with host molecules

摘要: Abstract Dental caries is the most common chronic infectious disease globally. The microbial communities associated with have mainly been examined using relatively low-resolution 16S rRNA gene amplicon sequencing and/or downstream analyses that are unsound for compositional nature of data provided by sequencing. Additionally, relationship between caries, oral microbiome composition, and host immunological markers has not explored. In this study, a panel 38 was analyzed across saliva from 23 children dentin 24 healthy dentition. Metagenomic sequencing, followed investigation tools designed to be robust data, illustrated several Prevotella spp. were prevalent in while Rothia health. contributional diversity (extent which multiple taxa contribute each pathway) functional pathways present decreased group. This decrease especially noticeable known impede pathogenesis, including arginine branched-chain amino acid biosynthesis. 10 found significantly elevated group, microbe-metabolite co-occurrence analysis an atlas relationships contributing bi-directional influence immune system. Finally, 527 metagenome-assembled genomes obtained metagenomics representing 151 species. novel genera/species further 20 study thus serves as model pipeline will tremendously expand our knowledge its dental once applied large populations.