Inhibition of proliferation and expression of AR/PSA by herbal supplement Equiguard in LNCaP cells cultured in androgen-proficient FBS and androgen-deficient charcoal-stripped FBS is correlated with increased serine-15 phosphorylation of the tumor suppressor gene p53

摘要: Use of dietary supplements and botanical products is widely accepted by patients diagnosed with prostate cancer (CaP) as a primary or complementary form treatment for their medical conditions in the U.S. Yet, majority these have not been rigorously studied regard to scientific mechanism(s). Because many available are mixtures multiple extracts derived from plants, some which necessarily native U.S., we consider mechanistic studies under defined laboratory be valuable essential, only standpoint standardization possible contamination products, but also providing insights evidence clinical efficacy purportedly demonstrate. In previous this laboratory, Equiguard, composite supplement consisting standardized nine Chinese herbs, was originally formulated correct physiological decline kidney functions associated age, fortuitously found display anti-CaP properties. Using panel CaP cells, showed that ethanol Equiguard significantly inhibited cell growth, induced apoptosis, lowered expression androgen receptor (AR), decreased intracellular secreted prostate-specific antigen (PSA) levels completely abolished colony forming activities cells. Since responsiveness observed cells mimicking androgen-dependent (AD) androgen-independent (AI) states CaP, our results raise interesting possibility herbal may potentially prevent, delay circumvent onset AI, thereby induce chronic instead terminal CaP. ablation therapy (chemical surgical castration) mainstay localized questioned whether might still exert aforementioned experimental settings modeled after ablation. Accordingly, effects LNCaP cultured androgen-proficient (FBS) -deficient (CS-FBS) media simulate hormonal status pre- post-castration vivo. Extracts were effective reducing formation, proliferation PCNA CS-FBS. Moreover, within concentration range genes, PSA AR, affected similar extent either FBS CS-FBS, correlated increased phosphorylation at serine-15 tumor suppressor gene p53. These consistent interpretation anti-proliferative modulatory properties largely independent androgens culture media.