Comparison of Extracellular Proteins from Virulent and Avirulent Vibrio parahaemolyticus Strains To Identify Potential Virulence Factors.

摘要: Vibrioparahaemolyticus is a leading seafood-borne pathogen that causes gastroenteritis, septicemia, and serious wound infections due to the actions of virulence-associated proteins. We compared extracellular proteins nonvirulent JHY20 virulent ATCC 33847 V. parahaemolyticus reference strains. Eighteen were identified from secretory profiles, 11 (68.75%) 16 in are associated with virulence and/or protection against adverse conditions: trigger factor, chaperone SurA, aspartate-semialdehyde dehydrogenase, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, glutamate 5-kinase, alanine glyceraldehyde-3-phosphate outer membrane protein OmpV, ribosome-associated inhibitor A, Skp, universal stress protein. Two nontoxic-related proteins, amino acid ABC transporter substrate-binding an uncharacterized protein, JHY20. The results provide theoretical basis for supporting safety risk assessment aquatic foods, illuminate pathogenic mechanisms parahaemolyticus, assist identification novel vaccine candidates foodborne pathogens.