HeLa cell nucleic acid metabolism. The effect of Mycoplasma contamination.
作者： E.H. Harley , K.R. Rees , A. Cohen
DOI: 10.1016/0005-2787(70)90018-3
关键词: Nucleic acid metabolism 、 Molecular biology 、 Ribosomal RNA 、 HeLa 、 Biology 、 Biochemistry 、 Extrachromosomal DNA 、 Nucleic acid 、 RNA 、 Uridine 、 Thymidine
摘要: Abstract 1. An altered pattern of RNA synthesis in a line normally growing HeLa cells has been studied. 2. Although low overall incorporation [ 3 H]uridine which failed to label the 45-S rRNA precursor characterised these cells, 14 C]methionine into showed its continued and normal maturation. 3. H]Uridine host cell labelled two newly appearing extranuclear species sedimenting on either side 18-S rRNA. 4. A DNA bouyant density was with H]thymidine. 5. The nucleic acids proved be similar those mycoplasma, mycoplasma contamination later confirmed by electron microscopic cultural techniques.
elsevier.com
sci-hub.se 参考文章(19)
Jonathan R. Warner, Ruy Soeiro, H.C. Birnboim, Marc Girard, James E. Darnell, Rapidly labeled HeLa cell nuclear RNA: I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA Journal of Molecular Biology. ,vol. 19, pp. 349- 361 ,(1966) , 10.1016/S0022-2836(66)80009-8
W.G. Flamm, H.E. Bond, H.E. Burr, Density-Gradient centrifugation of DNA in a fixed-angle rotor: A higher order of resolution Biochimica et Biophysica Acta. ,vol. 129, pp. 310- 317 ,(1966) , 10.1016/0005-2787(66)90373-X
Harold P. Morris, William B. Looney, Lillie O. Chang, Comparative Incorporation of Tritiated Thymidine and Cytidine into the Mitochondrial and Nuclear DNA and RNA of Two Transplantable Hepatomas (3924A and H-35tc2) and Host Livers Cancer Research. ,vol. 28, pp. 2164- 2167 ,(1968)
C.N.D. Cruickshank, J.R. Cooper, M.B. Conran, A new tissue culture chamber. Experimental Cell Research. ,vol. 16, pp. 695- 698 ,(1959) , 10.1016/0014-4827(59)90141-7
Klaus Scherrer, J.E. Darnell, Sedimentation characteristics of rapidly labelled RNA from HeLa cells Biochemical and Biophysical Research Communications. ,vol. 7, pp. 486- 490 ,(1962) , 10.1016/0006-291X(62)90341-8
Ernest F. Zimmerman, Barbara W. Holler, Methylation of 45 s ribosomal RNA precursor in HeLa cells Journal of Molecular Biology. ,vol. 23, pp. 149- 161 ,(1967) , 10.1016/S0022-2836(67)80023-8
BEATRIX MARKUS KOPRIWA, C. P. LEBLOND, IMPROVEMENTS IN THE COATING TECHNIQUE OF RADIOAUTOGRAPHY Journal of Histochemistry and Cytochemistry. ,vol. 10, pp. 269- 284 ,(1962) , 10.1177/10.3.269
S. Penman, RNA metabolism in the HeLa cell nucleus. Journal of Molecular Biology. ,vol. 17, pp. 117- 130 ,(1966) , 10.1016/S0022-2836(66)80098-0
George A. Bray, A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter Analytical Biochemistry. ,vol. 1, pp. 279- 285 ,(1960) , 10.1016/0003-2697(60)90025-7
Harold J. Morowitz, Hans R. Bode, R. Gary Kirk, The nucleic acids of mycoplasma. Annals of the New York Academy of Sciences. ,vol. 143, pp. 110- 114 ,(1967) , 10.1111/J.1749-6632.1967.TB27650.X