Chapter 15 Detection of Mycoplasma Contamination in Cultured Cells: Comparison of Biochemical, Morphological, and Microbiological Techniques

摘要: Publisher Summary This chapter describes the techniques employed in studies of detection mycoplasma contamination cultured cells. It focuses on two new biochemical techniques—namely, (1) identification labeled RNAs by polyacrylamide gel electrophoresis and (2) measurement incorporation exogenous bases nucleotides into RNA. The most commonly medium developed for growth is composed Difco beef heart infusion, Bacto Peptone NaCl supplemented with yeast extract horse agammaglobulin serum. introduction has led to clear separation 23 S E (Svedberg constant electrophoresis) 16 from host cell RNA species. comparative quantitative morphological emphasize difficulty screening cultures microbiological methods. Also, considering marked heterogeneity species their fastidious nutrient requirements, it possible that certain may be missed cultural testing.