The RNAs of Defective Interfering Pichinde Virus

摘要: Summary A Pichinde persistently infected BHK21/13S culture was established in which defective interfering (DI) virus continued to be synthesized after cessation of plaque-forming replication. This DI virus, concentrated from NaCl-polyethylene glycol treated tissue fluids, shown band over a much broader range than standard either discontinuous or continuous sucrose gradients. The polyacrylamide gel profile the RNAs extracted contained six components with sedimentation coefficients corresponding 31, 28, 22, 18, 15 and 4–6S. All preparations, however, did not contain 22 15S species. Furthermore, new 20S fraction observed taken cultures had been maintained for more 175 generations initial infection, whereas it absent prior that time.