VE1 immunohistochemistry predicts BRAF V600E mutation status and clinical outcome in colorectal cancer.

摘要: // Christian Schafroth 1 , Jose A. Galvan Irene Centeno Viktor H. Koelzer 1,2 Heather E. Dawson Lena Sokol Gregor Rieger Martin D. Berger 3 Marion Hadrich 4 Robert Rosenberg 5 Ulrich Nitsche 6 Beat Schnuriger Rupert Langer Daniel Inderbitzin 4,7 Alessandro Lugli and Inti Zlobec Translational Research Unit, Institute of Pathology, Bern University Hospital, Bern, Switzerland 2 Division Clinical Department Medical Oncology, Visceral Surgery Medicine, Surgery, Kantonsspital Baselland, Liestal, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany 7 Tiefenau Correspondence to: Zlobec, email: Keywords : colorectal cancer, BRAF, VE1, heterogeneity, prognosis, metastasis, Pathology Section Received September 22, 2015 Accepted October 04, Published 19, Abstract Aim: VE1 is a monoclonal antibody detecting mutant BRAF V600E protein by immunohistochemistry. Here we aim to determine the inter-observer agreement concordance with mutational status, investigate heterogeneity in cancers metastases prognostic effect cancer patients. Methods: Concordance status were tested on pilot cohort ( n = 34), melanomas 23) thyroid 8). Two cohorts evaluated 259, Cohort 226, 2) multiple-punch tissue microarrays. staining preoperative biopsies 118 patients) was compared expression resections. Primary tumors from 13 patients for using microarray generated all available blocks 100 blocks). Results: Inter-observer 100% (kappa 1.0). between mutation 98.5%. 1: positivity (seen 13.5%) associated older age p 0.0175) MLH1 deficiency < 0.0001). 2: 12.8%) female gender 0.0016), right-sided tumor location 0.0001), higher grade 0.0001) mismatch repair (MMR)-deficiency In survival analysis, MMR postoperative therapy identified as possible confounding factors. Adjusting these features, an unfavorable factor. Preoperative biopsy matched resections cases except one. No found across any primary/metastatic blocks. Conclusion: highly concordant homogeneously expressed suggesting can be analysed resection specimens, biopsies, metastatic lesions