Molecular Diagnosis of Ocular Infections

摘要: Molecular analysis of ocular fluids and biopsies has taken a prominent position in the diagnosis infections. Currently real-time PCR is mostly applied assay worldwide. In particular for viruses fastidious microorganisms, replaced culture since faster more sensitive. been used trachoma, herpes virus keratitis, infectious uveitis long time not only detection agent but also to monitor effect treatment. The application molecular tools bacterial fungal keratitis endophthalmitis taking flight. Panbacterial panfungal assays allow fast microorganism can be combined with subsequent species strain identification antibiotic resistance profiling. However, most current are expensive require costly well-equipped laboratories. Therefore, inexpensive point-of-care being developed. other diagnostic have their limitations. False-negative results may occur due absence analyzed sample which related type analyzed, sampling course disease, or merely nature infection. False-positive outcomes contamination bystander effects, resulting from ever-increasing sensitivities assays. When using tools, one should aware limitations consider as well.