Inheritance of polymorphic markers generated by DNA amplification fingerprinting and their use as genetic markers in soybean.
作者： Rekha R. Prabhu , Peter M. Gresshoff
DOI: 10.1007/BF00039524
关键词: Amplified fragment length polymorphism 、 Gene mapping 、 Mendelian inheritance 、 Population 、 Glycine soja 、 Biology 、 Genetics 、 DNA profiling 、 Molecular biology 、 GC-content 、 Genetic marker
摘要: DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs studied cross between the ancestral Glycine soja PI468.397 Merr. line nts382, F1 F2 progeny. reaction carried out with genomic 8 base long oligounucleotide primers. Silver-stained 5% polyacrylamide gels containing 7 M urea detected from 11 28 DAF products primers varying GC content (ranging 50 100% GC). Depending on their intensity, were classified into three classes. profiles reproducible for different extractions gels. Forty by 26 when comparing G. max. Most inherited as dominant Mendelian populations. However, abnormal occured about 25% polymorphisms. One marker maternal marker, presumably originating organelle while another showed apparent paternal inheritance. To confirm nuclear origin utility markers, polymorphisms mapped F11 mapping population recombinant inbred lines cultivars Minsoy × Noir 1. study that DAF-generated occur frequently reliably, they are loci can be genome mapping.
springer.com
sci-hub.se 参考文章(27)
Honeycutt Rl, Rittenhouse Kd, Ellsworth Dl, Artifactual variation in randomly amplified polymorphic DNA banding patterns. BioTechniques. ,vol. 14, pp. 214- ,(1993)
Scott V. Tingey, J. Antoni Rafalski, John G. K. Williams, Scott Sebastian, Soybean Genome Analysis: DNA Polymorphisms are Identified by Oligonucleotide Primers of Arbitrary Sequence Plant Molecular Biology 2. pp. 263- 268 ,(1991) , 10.1007/978-1-4615-3304-7_27
Benjamin Burr, Timothy Helentjaris, Development and Application of Molecular Markers to Problems in Plant Genetics ,(1989)
Donald Grierson, Simon N. Covey, The Plastome and Chloroplast Biogenesis Springer, Dordrecht. pp. 47- 77 ,(1988) , 10.1007/978-94-010-9649-2_3
John Welsh, Michael McClelland, Fingerprinting genomes using PCR with arbitrary primers Nucleic Acids Research. ,vol. 18, pp. 7213- 7218 ,(1990) , 10.1093/NAR/18.24.7213
R. J. Singh, T. Hymowitz, The genomic relationship between Glycine max (L.) Merr. and G. soja Sieb. and Zucc. as revealed by pachytene chromosome analysis. Theoretical and Applied Genetics. ,vol. 76, pp. 705- 711 ,(1988) , 10.1007/BF00303516
Gustavo Caetano-Anollés, Brant J. Bassam, Peter M. Gresshoff, DNA amplification fingerprinting: A strategy for genome analysis Plant Molecular Biology Reporter. ,vol. 9, pp. 294- 307 ,(1991) , 10.1007/BF02672006
Michael F. Riedy, William J. Hamilton, Charles F. Aquadro, Excess of non-parental bands in offspring from known primate pedigrees assayed using RAPD PCR Nucleic Acids Research. ,vol. 20, pp. 918- 918 ,(1992) , 10.1093/NAR/20.4.918
Brant J. Bassam, Gustavo Caetano-Anollés, Peter M. Gresshoff, Fast and sensitive silver staining of DNA in polyacrylamide gels Analytical Biochemistry. ,vol. 196, pp. 80- 83 ,(1991) , 10.1016/0003-2697(91)90120-I
Gustavo Caetano-Anollés, Bassam Brant J., Gresshoff Peter M., DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Nature Biotechnology. ,vol. 9, pp. 553- 557 ,(1991) , 10.1038/NBT0691-553