Influence on collagen metabolism of vitreous from eyes with proliferative vitreoretinopathy.

摘要: Purpose: Proliferative vitreoretinopathy ( P VR) is characterized by cell proliferation and membrane formation on the vitreoretinal cavity of eye. The membranes are composed extracellular matrix, mainly collagen type I. To explore possible mechanisms involved in PVR formation, authors analyzed role vitreous humor turnover. Methods: studied samples from ten patients with five donor eyes (keratoplasty) as control group. Human lung fibroblasts were used to study influence synthesis proliferation. Neutralizing antibodies against transforming growth factor-beta 2 (TGF-β ) inhibit fibroblast induced samples. Collagenolytic activity was fluid using 3 H-labeled collagen. Results: found that obtained significantly increased (2979 ± 963.26 versus 800 232 dpm 3Hproline incorporated per milligram vitreous-incubated protein; both groups (0 481 59 Hproline protein for samples, respectively). considerably lower derived compared group (19.9 20.3 234.1 19.1 ,μg degraded Conclusion: These results suggest a combined mechanism, including an increase mediated at least part TGF-02 decrease degradation, may contribute exaggerated deposition observed membranes, should be considered microenvironment participating actively pathogenesis this disorder.