Quantitative assessment of growth stimulating activity of the vitreous during PVR.

摘要: This report postulates that the activity of cellular proliferation along surface retina during proliferative vitreoretinopathy (PVR) is reflected in aggregate effect proliferation-inducing growth factors vitreous. A method for quantifying "net" capacity an individual vitreous sample was developed and this assay used to evaluate a model experimental PVR. Vitreous aspirated sequentially after onset fibroblastinduced PVR rabbit model. simple bioassay "aggregate" stimulating each assigned quantitative value terms "proliferation units" (PU). Experimental eyes demonstrated wide range (0-1765 PU), but control showed uniformly low levels (0-337 PU). ultimately retinal detachment displayed higher than did those destined remain attached. The differences were statistically different by day 3, prior clinical changes. We conclude quantification proliferation-stimulating possible might be useful screening at high risk development recurrent from Invest Ophthalmol Vis Sci 33:2436-2442, 1992 Proliferative remains leading cause persistent detachment, complicating as many 10% all detachments. 1 ' 2 Although pathogenesis complex, least four distinct stages appear important its development, including chemotaxis migration, proliferation, membrane formation, contraction. 3 " 8 Many studies are consistent with notion fluid/gel forms microenvironment reflects, if not modulates, these phases, especially PVR-inducing cells retina. 9 14 cavity, vitrectomized eye, can thought collecting pool produced proliferating leaking blood. Not surprisingly, investigators have stimulate