Pioneer study of population genetics of Rhodnius ecuadoriensis (Hemiptera: Reduviidae) from the central coastand southern Andean regions of Ecuador

摘要: Effective control of Chagas disease vector populations requires a good understanding the epidemiological components, including reliable analysis genetic structure populations. Rhodnius ecuadoriensis is most widespread in Ecuador, occupying domestic, peridomestic and sylvatic habitats. It widely distributed central coast southern highlands regions two very different terms bio-geographical characteristics. To evaluate relationship among R. these regions, we analyzed variability at microsatellite loci for 326 specimens (n=122 Manabi n=204 Loja) mitochondrial cytochrome b gene (Cyt b) sequences 174 individuals collected provinces (n=73 and=101 Loja respectively). The individual samples were grouped according to their community origin. A few presented positive FIS, possible due Wahlund effect. Significant pairwise differentiation was detected between within each province both markers, isolation by distance model significant Microsatellite markers showed provinces. partial Cyt (578bp) identified total 34 haplotypes sequenced, which translated into high haplotype diversity (Hd=0.929). distribution differed (significant Fisher's exact test). Overall, this study consistent with biological phenotypic differences previously observed current phylogenetic evidenced monophyly pallescens species complex; colombiensis more closely related than they ecuadoriensis.