Genetic factors influencing the regeneration ability of rye (Secale cereale L.). II. Immature embryos
作者： Monika Rakoczy-Trojanowska , Stefan Malepszy
DOI: 10.1007/BF01678135
关键词: Embryo culture 、 Genetic analysis 、 Inbred strain 、 Embryo 、 Andrology 、 Botany 、 Biology 、 Murashige and Skoog medium 、 Secale 、 Explant culture 、 Callus
摘要: Immature embryos of seven rye inbred lines were cultured on modified MS medium containing 3 mg/dm−3 2,4-D. According to thein vitro response divided into four groups: (1) those producing non-embryogenic callus (NEC) from above 30% the and having a high rate non-responding (NR) embryos, (2) majority (3) NEC by with percentage calli regenerating roots, (4) embryogenic (EC) plants 50% embryos. The inheritance these types was analysed in F1, F2, F3 generations crosses some lines. results obtained indicate that EC production both plant root regeneration are determined recessive genes whereas reduced ability for most probably dominant genes. lack is controlled at least two interacting
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