Preparation and characterization of poly(DL-lactide-co-glycolide) nanoparticles for siRNA delivery.
作者： Dongmei Cun , Camilla Foged , Mingshi Yang , Sven Frøkjær , Hanne Mørck Nielsen
DOI: 10.1016/J.IJPHARM.2009.10.023
关键词: Small interfering RNA 、 Nanotechnology 、 Double emulsion solvent evaporation 、 Combinatorial chemistry 、 Poly dl lactide co glycolide 、 Polymer 、 In vivo 、 Nanoparticle 、 Gene knockdown 、 Chemistry 、 PLGA
摘要: Abstract Synthetic short interfering RNA (siRNA) is promising for specific and efficient knockdown of disease-related genes. However, in vivo application siRNA requires an effective delivery system. Commonly used carriers are based on polycations, which form electrostatic complexes with siRNA. Such poly- or lipoplexes limited use due to severe problems associated toxicity, serum instability non-specific immune-responses. The aim the present study was prepare uniformly sized nanoparticles (NPs) a high load by safe biodegradable poly-( dl -lactide-co-glycolide) (PLGA) polymer without including polycations. encapsulated core NPs double emulsion solvent evaporation method. To optimize NP formulation, effects important formulation processing parameters were investigated systematically. Generally, spherical siRNA-loaded (
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Francisco Garcia, Karl Ulrich Petry, Laila Muderspach, Michael A Gold, Patricia Braly, Christopher P Crum, Marianne Magill, Michael Silverman, Robert G Urban, Mary Lynne Hedley, Kathleen J Beach, None, ZYC101a for Treatment of High-Grade Cervical Intraepithelial Neoplasia: A Randomized Controlled Trial Obstetrics & Gynecology. ,vol. 103, pp. 317- 326 ,(2004) , 10.1097/01.AOG.0000110246.93627.17
Swayam Prabha, Vinod Labhasetwar, Critical determinants in PLGA/PLA nanoparticle-mediated gene expression. Pharmaceutical Research. ,vol. 21, pp. 354- 364 ,(2004) , 10.1023/B:PHAM.0000016250.56402.99
Fuminori Ito, Hiroyuki Fujimori, Kimiko Makino, Factors affecting the loading efficiency of water-soluble drugs in PLGA microspheres. Colloids and Surfaces B: Biointerfaces. ,vol. 61, pp. 25- 29 ,(2008) , 10.1016/J.COLSURFB.2007.06.029
Rajesh Krishnamurthy, Janice A Lumpkin, Rajagopalan Sridhar, Inactivation of lysozyme by sonication under conditions relevant to microencapsulation. International Journal of Pharmaceutics. ,vol. 205, pp. 23- 34 ,(2000) , 10.1016/S0378-5173(00)00473-7
Kohei Tahara, Takeshi Sakai, Hiromitsu Yamamoto, Hirofumi Takeuchi, Yoshiaki Kawashima, Establishing chitosan coated PLGA nanosphere platform loaded with wide variety of nucleic acid by complexation with cationic compound for gene delivery International Journal of Pharmaceutics. ,vol. 354, pp. 210- 216 ,(2008) , 10.1016/J.IJPHARM.2007.11.002
Hongtao Lv, Shubiao Zhang, Bing Wang, Shaohui Cui, Jie Yan, Toxicity of cationic lipids and cationic polymers in gene delivery. Journal of Controlled Release. ,vol. 114, pp. 100- 109 ,(2006) , 10.1016/J.JCONREL.2006.04.014
Xudong Yuan, Ling Li, Appu Rathinavelu, Jinsong Hao, Madhusudhanan Narasimhan, Matthew He, Viviene Heitlage, Linda Tam, Sana Viqar, Mojgan Salehi, SiRNA drug delivery by biodegradable polymeric nanoparticles. Journal of Nanoscience and Nanotechnology. ,vol. 6, pp. 2821- 2828 ,(2006) , 10.1166/JNN.2006.436
Antonin R. de Fougerolles, Delivery vehicles for small interfering RNA in vivo. Human Gene Therapy. ,vol. 19, pp. 125- 132 ,(2008) , 10.1089/HUM.2008.928
Yogesh Patil, Jayanth Panyam, Polymeric nanoparticles for siRNA delivery and gene silencing International Journal of Pharmaceutics. ,vol. 367, pp. 195- 203 ,(2009) , 10.1016/J.IJPHARM.2008.09.039
Dana J. Gary, Nitin Puri, You-Yeon Won, Polymer-based siRNA delivery: Perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery Journal of Controlled Release. ,vol. 121, pp. 64- 73 ,(2007) , 10.1016/J.JCONREL.2007.05.021