Enzymatic hydrolysis of pantetheine.

摘要: Publisher Summary This chapter illustrates assay, purification, and properties of pantetheine. The enzymatic hydrolysis pantetheine to pantothenate cysteamine is the final common reaction in degradation coenzymes phosphopantetheine coenzyme A vitamin pantothenic acid. has been monitored by separation radiolabeled substrate product electrophoresis, pH-stat titration products, amino acid analysis N-ethylmaleimide adduct cysteamine. assay best used on tissue homogenates, whereas continuous mercaptide more convenient for partially purified preparations. Radiolabeled are separated a fast paper chromatography system quantified liquid scintillation counting. Activity nondenaturing polyacrylamide gels visualized local cresol red. produces at pH values intermediate pK enzyme activated thiols inhibited thiol inhibitors. glycoprotein containing 11.8% carbohydrate weight. Pantetheine hydrolase activity identified human fibroblasts white blood cells several organs mammalian species.