A study of the interactions of Hg(II) with T-T mispair containing hairpin loops
作者： Ajar Kamal , Zhe She , Renu Sharma , Heinz-Bernhard Kraatz
DOI: 10.1016/J.ELECTACTA.2017.05.058
关键词: Cooperative binding 、 Circular dichroism 、 Thermal analysis 、 Electrochemistry 、 Isothermal titration calorimetry 、 Chemistry 、 Dielectric spectroscopy 、 Analytical chemistry 、 Thermochemistry 、 Crystallography 、 Oligonucleotide
摘要: Abstract This manuscript describes the result of a series electrochemical, spectroscopic and thermodynamic studies into oligonucleotide hairpin structures equipped with T-T mispairs investigates their interaction Hg(II). For this purpose, three DNAs were designed, in which stem-region remains constant loop region contains increasing (5ʹ-HO-(CH 2 ) 6 -S-S-(CH -TGTAGTTCCTTCTACA-3ʹ, 5ʹ-HO-(CH -TGTAGTTTTCCTTTTCTACA-3ʹ; -TGTAGTTTTTTCCTTTTTTCTACA-3ʹ). Various techniques such as electrochemical impedance spectroscopy (EIS), circular dichroism (CD), UV-visible isothermal titration calorimetry (ITC) employed to get systematic evaluation need for multiple enhanced Hg(II) binding. EIS shows an increase charge transfer resistance (Δ R CT due structural changes within thin film caused by The CD indicate induced signals from right-handed left handed structures. Highest cooperative binding was observed DNA having maximum supplemented detailed thermal analysis DNA-Hg(II) These findings further supported measurements thermochemistry ITC. Our show that number are critical maximizing nucleobase-Hg(II) interactions.
sci-hub.se 参考文章(50)
Lanying Li, Yanli Wen, Li Xu, Qin Xu, Shiping Song, Xiaolei Zuo, Juan Yan, Weijia Zhang, Gang Liu, Development of mercury (II) ion biosensors based on mercury-specific oligonucleotide probes Biosensors and Bioelectronics. ,vol. 75, pp. 433- 445 ,(2016) , 10.1016/J.BIOS.2015.09.003
Ensheng Xu, Yanqin Lv, Jifeng Liu, Xiaohong Gu, Shuqiu Zhang, An electrochemical study based on thymine–Hg–thymine DNA base pair mediated charge transfer processes RSC Advances. ,vol. 5, pp. 49819- 49823 ,(2015) , 10.1039/C5RA06238A
Gaofeng Liu, Zhiwen Li, Junfei Zhu, Yang Liu, Ying Zhou, Junlin He, Studies on the thymine–mercury–thymine base pairing in parallel and anti-parallel DNA duplexes New Journal of Chemistry. ,vol. 39, pp. 8752- 8762 ,(2015) , 10.1039/C5NJ01859E
Hidetaka Torigoe, Yukako Miyakawa, Akira Ono, Tetsuo Kozasa, Positive cooperativity of the specific binding between Hg2+ ion and T:T mismatched base pairs in duplex DNA Thermochimica Acta. ,vol. 532, pp. 28- 35 ,(2012) , 10.1016/J.TCA.2011.03.018
Huijiao Sun, Xiaohong Li, Yunchao Li, Louzhen Fan, Heinz-Bernhard Kraatz, A novel colorimetric potassium sensor based on the substitution of lead from G-quadruplex The Analyst. ,vol. 138, pp. 856- 862 ,(2013) , 10.1039/C2AN36564B
Reed M. Izatt, James J. Christensen, J. Howard Rytting, Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and and nucleotides Chemical Reviews. ,vol. 71, pp. 439- 481 ,(1971) , 10.1021/CR60273A002
Dieter W. Gruenwedel, Michael K. Cruikshank, Mercury-induced DNA polymorphism: probing the conformation of Hg(II)-DNA via staphylococcal nuclease digestion and circular dichroism measurements. Biochemistry. ,vol. 29, pp. 2110- 2116 ,(1990) , 10.1021/BI00460A021
Axel Walter, Gerhard Luck, Interactions of Hg(II) ions with DNA as revealed by CD measurements Nucleic Acids Research. ,vol. 4, pp. 539- 550 ,(1977) , 10.1093/NAR/4.3.539
Shana O. Kelley, Jacqueline K. Barton, DNA-mediated electron transfer from a modified base to ethidium: π-stacking as a modulator of reactivity Chemistry & Biology. ,vol. 5, pp. 413- 425 ,(1998) , 10.1016/S1074-5521(98)90158-2
Xuejia Xue, Feng Wang, Xiaogang Liu, One-step, room temperature, colorimetric detection of mercury (Hg2+) using DNA/nanoparticle conjugates. Journal of the American Chemical Society. ,vol. 130, pp. 3244- 3245 ,(2008) , 10.1021/JA076716C