A microfluidic immunoassay system on a centrifugal platform

摘要: Abstract A lab-on-a-CD immunoassay system was designed, fabricated, and tested. The actuator for the centrifugal platform consists of a flyball governor group spring plungers. microfluidic structures were fabricated using replica processes with poly-dimethylsiloxane (PDMS) as structural material. For better control, valves in designed to be mechanical-pinch ones actuated. burst frequencies micro-valves can tuned independently by adjusting heights pre-stresses corresponding research work reported this paper, four sequential one inward pump integrated on develop an automated system. Mouse IgG used model analyte test feasibility proposed device applications. Sample solution goat anti-mouse conjugated magnetic nanoparticles trapping mechanism eliminate blocking process. Rabbit labeled fluorescent molecules signal reporter. entire assay process, including beads trapping, reaction, washing pumping completed platform. detection sensitivity may also improved higher quality monoclonal antibodies. This new offers some significant advantages over those field: simple fabrication, robust easy integration, lower cost. modified multiple targets. technology has great potential applications complex fluid handling needed biological, chemical medical application Lab-on-a-CD systems.