A complex array of double-stranded and single-stranded DNA-binding proteins mediates induction of the ovalbumin gene by steroid hormones

摘要: Abstract The transcriptional induction of the chicken ovalbumin gene by steroid hormones is abolished inhibitors protein synthesis such as cycloheximide, suggesting that a labile mediates this process. A steroid-dependent regulatory element (SDRE) has been identified in 5'-flanking region between -900 and -780 required for steroids. Additional transfection experiments limit 5'-border SDRE to -892 -864. To investigate whether any proteins binding are affected estrogen or was investigated using DNase I exonuclease III footprinting gel mobility shift assays. These demonstrate bind sequences -860 -810 -820. Four oviduct nuclear proteins, including one prefer single-stranded DNA (ssDNA) sequence-specific manner. activity three these ssDNA-binding increased extracts from estrogen-treated chicks. data suggest mediated complex collection ssDNA- double-stranded DNA-binding whose activities turn regulated their short half-lives estrogen.