Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation, and cross-species antiviral activity.

摘要: Abstract The finding that human leukocyte interferon preparations could be completely renatured after boiling in sodium dodecyl sulfate (SDS) but only partially reactivated SDS under reducing conditions suggested interferons contain two types of interferons. Here we report do, indeed, molecular populations can distinguished physically, chemically, and biologically. Leukocyte boiled was electrophoresed SDS-polyacrylamide gels, peaks activity were obtained, a major peak at approximately 15,000 daltons minor 21,000 daltons. gels the peak, daltons, obtained: amount recovered identical whether samples reduced or not, eliminated by reduction. also exhibited disparity cross-reactivity on rabbit cells: population with weight active to same extent diploid cells secondary kidney cells, whereas expressed about 5% its homologous titer cells.