Gene and mRNA for precursor polypeptide VI from adenovirus type 2.

摘要: We present a 1,040-base-pair-long sequences of adenoviruses type 2 DNA which encodes the complete gene for precursor polypeptide VI (pVI). pVI consists 250 amino acids amounting to molecular weight 26,990. The proteolytic cleavage maturing virion removes 33 from amino-terminal end polypeptide, thus giving mature 23,400. UAA stop codon terminating translation is separated by 84 nucleotides initiator triplet hexon gene. Both polypeptides are encoded same translational reading frame, suggesting evolution and as separate proteins introduction termination selection new splice acceptor site in an ancestral fused chain. where common tripartite leader attached mRNA precedes one nucleotide forms, together with other late sites, adenovirus consensus site. also demonstrate that 3' mRNA's belonging L2 3'-cotermination family located only 31 upstream junction mRNA. Furthermore, we show four novel weights 80,000, 39,000, 36,000, 10,500 region L2.