PROPERTIES OF VOLTAGE-GATED CURRENTS OF MICROGLIA DEVELOPED USING MACROPHAGE COLONY-STIMULATING FACTOR

摘要: Microglia were isolated from a murine neonatal brain cell culture in which their development had been stimulated by supplementation with the macrophage/microglial growth factor macrophage colony-stimulating (M-CSF). Using whole-cell configuration of patch-clamp technique, voltage-gated membrane currents recorded these microglial cells. Hyperpolarization induced inward rectifying K+ currents, as described for microglia untreated cultures. These activated negative to equilibrium potential and, strong hyperpolarization, displayed time-dependent inactivation. The inactivation was abolished when extracellular NaCl replaced N-methyl-d-glucamine (NMG), thereby indicating partial block this conductance Na+. Inward also blocked extracellularly applied Cs+ or Ba2+. They slightly diminished following treatment tetraethylammonium chloride (TEA) but not affected 4-aminopyridine (4-AP). Upon long lasting depolarizing voltage pulses potentials positive 0 mV, cells exhibited slowly activating H+ current could be reduced application inorganic polyvalent cations (Ba2+, Cd2+, Co2+, La3+, Ni2+, Zn2+) well 4-AP TEA. Based on kinetics and pharmacological characteristics, both detected M-CSF-grown are suggested correspond rectifier macrophages.