Endothelins, peptides with potent vasoactive properties, are produced by human macrophages.

摘要: Endothelins are peptides, originally isolated from endothelial cells, with potent vasoactive and mitogenic properties. In this study, we demonstrate that human macrophages synthesize secrete endothelins. Cultured were found by immunocytochemistry to stain positively for endothelin 1 3. Their capability produce release these peptides was confirmed a combination of reverse-phase high-performance liquid chromatography radioimmunoassays, specific 3, respectively. Immunoreactive identified both in cellular extracts macrophage-conditioned medium. The secretion 1, but not could be stimulated 6-10-fold lipopolysaccharide or phorbol myristate acetate (PMA). Northern blot analysis total macrophage RNA using an cDNA probe revealed induction mRNA PMA-treated macrophages. Furthermore, immunoreactive 3 U937 promonocytic line, freshly monocytes. contrast, no detected cell neutrophils lymphocytes. expression endothelins tissue demonstrated paraffin sections lung immunohistochemistry. conclusion, the finding suggests important role microenvironment Macrophage-derived may have essential function blood vessel physiology, aberrant production contribute pathology.