Optimization of growth and secretion of human monoclonal antibodies by hybridomas cultured in serum-free media.
作者： ALOIS B. LANG , ULRICH SCHUERCH , STANLEY J. CRYZ
关键词: Secretion 、 Transferrin 、 Cell culture 、 Microbiology 、 Antibody 、 Molecular biology 、 Serotype 、 Biology 、 Bacteria 、 Monoclonal antibody 、 Lipopolysaccharide
摘要: To facilitate the production and purification of human monoclonal antibodies, we evaluated ability hybridomas to adapt chemically defined-serum free media. From a panel secreting antibody against serotype specific lipopolysaccharide determinants gram-negative bacteria, growth secretion properties two producing antibodies strains Pseudomonas aeruqinosa, 4-8KH15 4-10KH139, were analysed. Both clones did not grow in protein-free medium. However, it was possible them serum-free media consisting basal medium supplemented with insulin, transferrin, ethanolamine, selenite. Antibody rates equal (4-8KH15: 26-31 μg IgM/106 cells/day) or higher (4-10KH139: 58-90 as compared conventional serum-supplemented Our studies suggest that adaptation described selected results an which is very hi...
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