Hybridoma cell growth and anti-neuroblastoma monoclonal antibody production in spinner flasks using a protein-free medium with microcarriers.

摘要: The main disadvantages of foetal calf serum as the world-wide common supplement for cell growth are its content various proteins variable concentrations between batches well high cost. use serum-free and protein-free media is gradually becoming one goals culture especially standardizing conditions or simple purification products like monoclonal antibodies. mouse hybridoma cells 14/2/1 were cultivated either in UltraDOMA medium serum-containing RPMI with without microcarriers to generate quantities antibodies against neuroblastoma tumour cells. Cell rate, IgG production, viability, glucose lactate concentrations, attachment rate doubling time have been used investigation criteria. Modifications procedures (static stirred), inoculum density, microcarrier concentration caused an improvement antibody production. kinetics synthesis was best spinner 2 ml medium. These results short-term stirred flasks indicate that yields 2.5-fold higher those serum-supplemented can be achieved.