Biosynthesis of Colabomycin E, a New Manumycin-Family Metabolite, Involves an Unusual Chain-Length Factor
作者： Kateřina Petříčková , Stanislav Pospíšil , Marek Kuzma , Tereza Tylová , Michal Jágr
关键词: Biosynthesis 、 Biochemistry 、 Stereochemistry 、 Gene cluster 、 Polyketide 、 Streptomyces 、 Metabolite 、 Streptomyces aureus 、 Biological activity 、 Biology 、 Recombinant DNA
摘要: Colabomycin E is a new member of the manumycin-type metabolites produced by strain Streptomyces aureus SOK1/5-04 and identified genetic screening from library streptomycete strains. The structures colabomycin accompanying congeners were resolved. entire biosynthetic gene cluster was cloned expressed in lividans. Bioinformatic analysis mutagenic studies components pathway that are involved formation both polyketide chains. Recombinant synthases (PKSs) assembled asukamycin routes catalyzing biosynthesis "lower" carbon chains constructed S. ΔcolC11-14 deletion mutant. Analysis recombinant strains provided evidence pathways length lower chain controlled an unusual chain-length factor supporting either triketide or tetraketide E. Biological activity assays indicated significantly inhibited IL-1β release THP-1 cells might thus potentially act as anti-inflammatory agent.
sci-hub.se 参考文章(50)
Ben Shen, Biosynthesis of Aromatic Polyketides Springer Berlin Heidelberg. pp. 1- 51 ,(2000) , 10.1007/3-540-48146-X_1
S. Pospíšil, K. Petříčková, P. Sedmera, P. Halada, J. Olšovská, M. Petříček, Effect of starter unit availability on the spectrum of manumycin-type metabolites produced by Streptomyces nodosus ssp. asukaensis. Journal of Applied Microbiology. ,vol. 111, pp. 1116- 1128 ,(2011) , 10.1111/J.1365-2672.2011.05132.X
Bertolt Gust, Govind Chandra, Dagmara Jakimowicz, Tian Yuqing, Celia J. Bruton, Keith F. Chater, λ Red-Mediated Genetic Manipulation of Antibiotic-Producing Streptomyces Advances in Applied Microbiology. ,vol. 54, pp. 107- 128 ,(2004) , 10.1016/S0065-2164(04)54004-2
Bradley S Moore, Christian Hertweck, Biosynthesis and attachment of novel bacterial polyketide synthase starter units Natural Product Reports. ,vol. 19, pp. 70- 99 ,(2002) , 10.1039/B003939J
Meilian Liu, Lijun Zhou, Aimin Xu, Karen SL Lam, Michael D Wetzel, Ruihua Xiang, Jingjing Zhang, Xiaoban Xin, Lily Q Dong, Feng Liu, A disulfide-bond A oxidoreductase-like protein (DsbA-L) regulates adiponectin multimerization Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 18302- 18307 ,(2008) , 10.1073/PNAS.0806341105
Abhirup Das, Chaitan Khosla, None, Biosynthesis of Aromatic Polyketides in Bacteria Accounts of Chemical Research. ,vol. 42, pp. 631- 639 ,(2009) , 10.1021/AR8002249
Jeffrey W. Nelson, Thomas E. Creighton, Reactivity and ionization of the active site cysteine residues of DsbA, a protein required for disulfide bond formation in vivo. Biochemistry. ,vol. 33, pp. 5974- 5983 ,(1994) , 10.1021/BI00185A039
R BALTZ, Renaissance in antibacterial discovery from actinomycetes. Current Opinion in Pharmacology. ,vol. 8, pp. 557- 563 ,(2008) , 10.1016/J.COPH.2008.04.008
Stephen R. Shouldice, Begoña Heras, Patricia M. Walden, Makrina Totsika, Mark A. Schembri, Jennifer L. Martin, Structure and function of DsbA, a key bacterial oxidative folding catalyst. Antioxidants & Redox Signaling. ,vol. 14, pp. 1729- 1760 ,(2011) , 10.1089/ARS.2010.3344
Miroslav Petříček, Kateřina Petříčková, Libor Havlíček, Jürgen Felsberg, Occurrence of Two 5-Aminolevulinate Biosynthetic Pathways in Streptomyces nodosus subsp. asukaensis Is Linked with the Production of Asukamycin Journal of Bacteriology. ,vol. 188, pp. 5113- 5123 ,(2006) , 10.1128/JB.01919-05