A disulfide-bond A oxidoreductase-like protein (DsbA-L) regulates adiponectin multimerization

摘要: Impairments in adiponectin multimerization lead to defects secretion and function are associated with diabetes, yet the underlying mechanisms remain largely unknown. We have identified an adiponectin-interacting protein, previously named GST-kappa, by yeast 2-hybrid screening. The protein contains 2 thioredoxin domains has very little sequence similarity other GST isoforms. However, this shares high secondary structure homology bacterial disulfide-bond A oxidoreductase (DsbA) is thus renamed DsbA-like (DsbA-L). DsbA-L highly expressed adipose tissue, its expression level negatively correlated obesity mice humans. 3T3-L1 adipocytes stimulated insulin sensitizer rosiglitazone inhibited inflammatory cytokine TNFα. Overexpression of promoted while suppressing RNAi markedly selectively reduced levels adipocytes. Our results identify as a key regulator for biosynthesis uncover potential new target developing therapeutic drugs treatment resistance metabolic disorders.