Luminex-based virtual crossmatching for renal transplantation in South Africa.

摘要: Background. Current practice in the Johannesburg renal transplantation programme is to perform a transplant when patient’s complement-dependent cytotoxicity and flow cytometric crossmatches are negative. However, even patients with negative early graft rejections have occurred. We retrospectively evaluated use of Luminex anti-human leukocyte antigen (HLA) antibody detection technology, often termed ‘virtual crossmatching’, compared crossmatch, for predicting outcome patients. Methods. Sixty-four recipients were crossmatched against multiple donors during their routine work-up (111 crossmatches); 17 these received transplants study period. Anti-HLA was performed using technology results crossmatch short-term success. Results. Compared results, sensitivity specificity virtual crossmatching 85.7% 90.7% T-cell 100% 87.2% B-cell crossmatch. Both success 100%. Conclusions. Strong evidence provided that single-antigen assays provide improved detect clinically relevant anti-HLA antibodies can reliably be used predict shortterm recommend incorporation methodology into algorithm South Africa.