Sequential analysis of HLA-class II antigen expression in human renal allografts. Induction of tubular class II antigens and correlation with clinical parameters.

摘要: The expression of HLA-class II antigens was assessed in 142 biopsies from 29 recipients cadaveric renal allografts who received either short-term cyclosporine (n = 12) or azathioprine and low-dose prednisolone 17). Biopsies were obtained before transplantation, routinely at days 7, 21, 90, 365 after other times as clinically indicated. Cryostat sections labeled with monoclonal antibodies using an indirect immunoperoxidase technique. In all expressed on glomeruli intertubular structures. pretransplant proximal tubules class antigens, whereas distal always negative. After transplantation three patterns recognized based tubular staining: normal, focal increased, generalized increased expression. Sequential biopsy analysis showed fluctuating levels individual patients, which correlated cellular infiltration associated allograft rejection. 71% day 90 patients conventional therapy compared only 9% immunosuppression. All normal antigen had well-functioning grafts two years 3 9 failed. Furthermore, failing irreversible rejection marked increase may be merely a marker have role the augmentation response, its induction target for effector arm reaction.