Interaction of echinomycin with guanine: electrochemistry and spectroscopy studies.
作者： Nan Li , Liping Guo , Junguang Jiang , Xiurong Yang
DOI: 10.1016/J.BPC.2004.06.007
关键词: Cyclic voltammetry 、 Ultraviolet visible spectroscopy 、 Electrochemistry 、 Photochemistry 、 Infrared spectroscopy 、 Chemistry 、 Hydrogen bond 、 Inorganic chemistry 、 Guanine 、 Echinomycin 、 Adduct
摘要: The interaction of antitumor antibiotic, echinomycin (Echi) with guanine (Gua) was thoroughly investigated by adsorptive transfer stripping cyclic voltammetry, ultraviolet and visible adsorption spectra (UV/Vis) Fourier-transform infrared spectroscopy (FTIR). Electrochemistry provided a simple tool for verifying the occurrence between Echi Gua. could be accumulated from solution give well-defined electrochemical signals in 0.1 M phosphate buffer (pH 7.0) only when Gua present on surface electrochemically pretreated glass carbon electrode (GCE), suggesting strong binding to All acquired spectral data showed that new adduct formed, two pairs adjacent intermolecular hydrogen bonds Ala backbone atoms (Ala-NH Gua-N3 Gua-NH2 Ala-CO) played dominating role interaction. coupled techniques provide relatively easy way obtain useful insights into molecular mechanism drug-DNA interactions, which should important development anticancer drugs specific base recognition.
core.ac.uk
irgrid.ac.cn
elsevier.com参考文章(40)
M. J. Waring, Echinomycin, Triostin, and Related Antibiotics Springer, Berlin, Heidelberg. pp. 173- 194 ,(1979) , 10.1007/978-3-642-46407-2_7
M. J. Waring, The Molecular Basis of Specific Recognition between Echinomycin and DNA Springer Netherlands. pp. 225- 245 ,(1990) , 10.1007/978-94-011-3728-7_16
R. K. Gilpin, C. S. Gilpin, Pharmaceuticals and related drugs. Analytical Chemistry. ,vol. 61, pp. 3755- 3770 ,(1975) , 10.1021/AC070708X
Zhenxin Wang, Dianjun Liu, Shaojun Dong, In situ infrared spectroelectrochemical studies on adsorption and oxidation of nucleic acids at glassy carbon electrode. Bioelectrochemistry. ,vol. 53, pp. 175- 181 ,(2001) , 10.1016/S0302-4598(00)00123-9
Jean Louis Leroy, Xiaolian Gao, Vinod Misra, Maurice Gueron, Dinshaw J. Patel, Proton exchange in DNA-luzopeptin and DNA-echinomycin bisintercalation complexes: rates and processes of base-pair opening. Biochemistry. ,vol. 31, pp. 1407- 1415 ,(1992) , 10.1021/BI00120A017
Christian Bailly, François Hamy, Michael J. Waring, Cooperativity in the Binding of Echinomycin to DNA Fragments Containing Closely Spaced CpG Sites† Biochemistry. ,vol. 35, pp. 1150- 1161 ,(1996) , 10.1021/BI951696P
Christopher MA Brett, Ana Maria Oliveira Brett, Silvia HP Serrano, None, On the adsorption and electrochemical oxidation of DNA at glassy carbon electrodes Journal of Electroanalytical Chemistry. ,vol. 366, pp. 225- 231 ,(1994) , 10.1016/0022-0728(93)02994-S
Eleanore Seibert, Alexander S. Chin, Wolfgang Pfleiderer, Mary E. Hawkins, William R. Laws, Roman Osman, J. B. Alexander Ross, pH-Dependent Spectroscopy and Electronic Structure of the Guanine Analogue 6,8-Dimethylisoxanthopterin † Journal of Physical Chemistry A. ,vol. 107, pp. 178- 185 ,(2003) , 10.1021/JP026904J
G. Quigley, G Ughetto, G. van der Marel, J. van Boom, A. Wang, A Rich, Non-Watson-Crick G.C and A.T base pairs in a DNA-antibiotic complex. Science. ,vol. 232, pp. 1255- 1258 ,(1986) , 10.1126/SCIENCE.3704650
Alvin L. Beilby, Tania A. Sasaki, Howard M. Stern, Electrochemical Pretreatment of Carbon Electrodes as a Function of Potential, pH, and Time Analytical Chemistry. ,vol. 67, pp. 976- 980 ,(1995) , 10.1021/AC00101A027